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Unique features of the grapevine VvK5.1 channel support novel functions for outward K+ channels in plants.

Authors
  • Villette, Jérémy1
  • Cuéllar, Teresa2, 3
  • Zimmermann, Sabine D1
  • Verdeil, Jean-Luc2, 3
  • Gaillard, Isabelle1
  • 1 BPMP, Université Montpellier, CNRS, INRA, SupAgro, Montpellier, France. , (France)
  • 2 CIRAD, UMR AGAP, F-34398 Montpellier, France. , (France)
  • 3 Université Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France. , (France)
Type
Published Article
Journal
Journal of Experimental Botany
Publisher
Oxford University Press
Publication Date
Nov 18, 2019
Volume
70
Issue
21
Pages
6181–6193
Identifiers
DOI: 10.1093/jxb/erz341
PMID: 31327013
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Grapevine (Vitis vinifera L.), one of the most important fruit crops, is a model plant for studying the physiology of fleshy fruits. Here, we report on the characterization of a new grapevine Shaker-type K+ channel, VvK5.1. Phylogenetic analysis revealed that VvK5.1 belongs to the SKOR-like subfamily. Our functional characterization of VvK5.1 in Xenopus oocytes confirms that it is an outwardly rectifying K+ channel that displays strict K+ selectivity. Gene expression level analyses by real-time quantitative PCR showed that VvK5.1 expression was detected in berries, roots, and flowers. In contrast to its Arabidopsis thaliana counterpart that is involved in K+ secretion in the root pericycle, allowing root to shoot K+ translocation, VvK5.1 expression territory is greatly enlarged. Using in situ hybridization we showed that VvK5.1 is expressed in the phloem and perivascular cells of berries and in flower pistil. In the root, in addition to being expressed in the root pericycle like AtSKOR, a strong expression of VvK5.1 is detected in small cells facing the xylem that are involved in lateral root formation. This fine and selective expression pattern of VvK5.1 at the early stage of lateral root primordia supports a role for outward channels to switch on cell division initiation. © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.

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