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Uncoupling of EphA/ephrinA signaling and spontaneous activity in neural circuit wiring.

Authors
  • Benjumeda, Isabel
  • Escalante, Augusto
  • Law, Chris
  • Morales, Daniel
  • Chauvin, Geraud
  • Muça, Gerald
  • Coca, Yaiza
  • Márquez, Joaquín
  • López-Bendito, Guillermina
  • Kania, Artur
  • Martínez, Luis
  • Herrera, Eloísa
Type
Published Article
Journal
Journal of Neuroscience
Publisher
Society for Neuroscience
Publication Date
Nov 13, 2013
Volume
33
Issue
46
Pages
18208–18218
Identifiers
DOI: 10.1523/JNEUROSCI.1931-13.2013
PMID: 24227729
Source
Medline
License
Unknown

Abstract

Classic studies have proposed that genetically encoded programs and spontaneous activity play complementary but independent roles in the development of neural circuits. Recent evidence, however, suggests that these two mechanisms could interact extensively, with spontaneous activity affecting the expression and function of guidance molecules at early developmental stages. Here, using the developing chick spinal cord and the mouse visual system to ectopically express the inwardly rectifying potassium channel Kir2.1 in individual embryonic neurons, we demonstrate that cell-intrinsic blockade of spontaneous activity in vivo does not affect neuronal identity specification, axon pathfinding, or EphA/ephrinA signaling during the development of topographic maps. However, intrinsic spontaneous activity is critical for axon branching and pruning once axonal growth cones reach their correct topographic position in the target tissues. Our experiments argue for the dissociation of spontaneous activity from hard-wired developmental programs in early phases of neural circuit formation.

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