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Ultraviolet photodissociation of chromophore-labeled oligosaccharides via reductive amination and hydrazide conjugation.

Authors
Type
Published Article
Journal
Organic Mass Spectrometry
1096-9888
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
46
Issue
4
Pages
359–366
Identifiers
DOI: 10.1002/jms.1901
PMID: 21438085
Source
Medline
License
Unknown

Abstract

The fragmentation patterns of hydrazide-conjugated and reductively aminated oligosaccharides, including lacto-N-fucopentaoses and lacto-N-difucohexaoses, produced on collisionally induced dissociation (CID) and ultraviolet photodissociation (UVPD) in a quadrupole ion trap are presented. The two derivatization methods generate different cross-ring cleavages on UVPD and CID. UVPD of hydrazide-conjugated oligosaccharides yield predominant (2, 4)A-type cross-ring cleavage ions. In contrast, UVPD of aminated oligosaccharides results mainly in (0, 1)A-type ions. Moreover, more extensive dual-cleavage pathways (i.e. internal fragment ions) were observed on UVPD.

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