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ULTRAVIOLET MICROSCOPY OF BUDDING SACCHAROMYCES

Authors
  • Dan O. McClary
  • Wilbert D. Bowers
  • Glendon R. Miller
Publication Date
Feb 01, 1962
Source
PMC
Keywords
License
Unknown

Abstract

McClary, Dan O. (Southern Illinois University, Carbondale), Wilbert D. Bowers, Jr., and Glendon R. Miller. Ultraviolet microscopy of budding Saccharomyces. J. Bacteriol. 83:276–283. 1962.—Synchronous cell division was obtained in Saccharomyces by transferring starved cells into nutrient medium. Ultraviolet microscopy and Giemsa-stained preparations of these cells showed nuclear division to occur in the mother cell early in the budding process. The divided nucleus passed into the neck between the mother cell and the bud, and either fused together again or the two parts became so closely associated that one continuous dumbbell-shaped body was seen which seemed to divide by constriction. This effect was probably due to the retention of the intact nuclear membrane until nuclear division was otherwise complete.

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