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Ultrastructure of guinea pig stria vascularis processed by rapid freezing and freeze substitution.

Authors
  • Kawaguchi, H
  • Arima, T
  • Uemura, T
Type
Published Article
Journal
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
Publication Date
Jan 01, 1994
Volume
251 Suppl 1
Identifiers
PMID: 11894772
Source
Medline
License
Unknown

Abstract

The rapid-freezing and freeze-substitution method fixes a specimen as if it were prepared before excision. We used this method to study the stria vascularis of guinea pigs using electron microscopy. Findings were essentially the same as those obtained with conventional chemical fixation, although freeze substitution made it possible to observe the membrane structures in a smoother and more linear manner. This method seems to be the procedure of choice for studying the instantaneous movement and behavior of cells.

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