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The ultrastructure of Chlorobaculum tepidum revealed by cryo-electron tomography.

Authors
  • Kudryashev, Misha1
  • Aktoudianaki, Aikaterini2
  • Dedoglou, Dimitrios2
  • Stahlberg, Henning3
  • Tsiotis, Georgios4
  • 1 Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, 4058 Basel, Switzerland; Focal Area Infection Biology, Biozentrum, University of Basel, 4058 Basel, Switzerland. , (Switzerland)
  • 2 Division of Biochemistry, Department of Chemistry, University of Crete, 71003 Voutes, Heraklion, Greece. , (Greece)
  • 3 Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, 4058 Basel, Switzerland. , (Switzerland)
  • 4 Division of Biochemistry, Department of Chemistry, University of Crete, 71003 Voutes, Heraklion, Greece. Electronic address: [email protected] , (Greece)
Type
Published Article
Journal
Biochimica et Biophysica Acta
Publisher
Elsevier
Publication Date
Oct 01, 2014
Volume
1837
Issue
10
Pages
1635–1642
Identifiers
DOI: 10.1016/j.bbabio.2014.06.002
PMID: 24950126
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Chlorobaculum (Cba) tepidum is a green sulfur bacterium that oxidizes sulfide, elemental sulfur, and thiosulfate for photosynthetic growth. As other anoxygenic green photosynthetic bacteria, Cba tepidum synthesizes bacteriochlorophylls for the assembly of a large light-harvesting antenna structure, the chlorosome. Chlorosomes are sac-like structures that are connected to the reaction centers in the cytoplasmic membrane through the BChl α-containing Fenna-Matthews-Olson protein. Most components of the photosynthetic machinery are known on a biophysical level, however, the structural integration of light harvesting with charge separation is still not fully understood. Despite over two decades of research, gaps in our understanding of cellular architecture exist. Here we present an in-depth analysis of the cellular architecture of the thermophilic photosynthetic green sulfur bacterium of Cba tepidum by cryo-electron tomography. We examined whole hydrated cells grown under different electron donor conditions. Our results reveal the distribution of chlorosomes in 3D in an unperturbed cell, connecting elements between chlorosomes and the cytoplasmic membrane and the distribution of reaction centers in the cytoplasmic membrane. Copyright © 2014 Elsevier B.V. All rights reserved.

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