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Ultrastructural analysis of v-myb oncogene product cooperation with components of avian cell nuclear matrix.

Authors
  • Korb, J
  • Stokrová, J
  • Karafiát, V
Type
Published Article
Journal
Folia biologica
Publication Date
Jan 01, 2000
Volume
46
Issue
4
Pages
137–142
Identifiers
PMID: 10954158
Source
Medline
License
Unknown

Abstract

The cooperation of the v-Myb oncoprotein with extracted nuclear matrix of avian haematopoietic cells expressing the v-myb oncogene was studied by means of immunoelectron microscopy. The nuclear matrix was extracted by a gentle method of detergent treatment at moderate ionic strength and visualized either in ultrathin LR White sections, in unembedded resin-free sections, and in addition by the aqueous spreading technique. Using anti-Myb polyclonal antibody we have shown interaction of the v-Myb protein product with extracted nuclear matrix. This oncoprotein, however, was easily released from the structure by a detergent as well as by DNAase treatment and ammonium sulphate extraction. Prefixation of structures before detergent treatment prevented this extraction. The v-Myb protein marker was distributed in clusters or associated with fibrillar structures in most cases. Single markers decorating these fibrillar or less dense structures were also detected.

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