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Ultrastructural analysis of low-threshold mechanoreceptive vibrissa afferent boutons in the cat trigeminal caudal nucleus.

Authors
  • Paik, Sang Kyoo1
  • Choi, Seung Ki
  • Lee, Jong Wook
  • Kim, Tae Heon
  • Ahn, Dong Kuk
  • Yoshida, Atsushi
  • Kim, Yun Sook
  • Bae, Yong Chul
  • 1 Department of Oral Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Daegu, Korea. , (North Korea)
Type
Published Article
Journal
Anatomy & cell biology
Publication Date
Dec 01, 2010
Volume
43
Issue
4
Pages
340–346
Identifiers
DOI: 10.5115/acb.2010.43.4.340
PMID: 21267409
Source
Medline
Keywords
License
Unknown

Abstract

Ultrastructural parameters related to synaptic release and their correlation with synaptic connectivity were analyzed in the low-threshold mechanoreceptive vibrissa afferent boutons in laminae III and IV of the trigeminal caudal nucleus (Vc). Rapidly adapting vibrissa afferents were intra-axonally labeled, and quantitative ultrastructural analyses with serial sections were performed on the labeled boutons and their presynaptic endings (p-endings). The volume of the labeled boutons was widely distributed from small to large ones (0.8~12.3 µm(3)), whereas the p-endings were small and uniform in size. The volume of the labeled boutons was positively correlated with the ultrastructural parameters such as mitochondrial volume (correlation coefficient, r=0.96), active zone area (r=0.82) and apposed surface area (r=0.79). Vesicle density (r=-0.18) showed little correlation to the volume of labeled boutons, suggesting that the total vesicle number of a bouton is proportional to its volume. In addition, the bouton volume was positively correlated with the number of p-endings (r=0.52) and with the number of dendrites postsynaptic to the labeled bouton (r=0.83). These findings suggest that low-threshold mechanoreception conveyed through vibrissa afferents is processed in a bouton size-dependent manner in the Vc, which may contribute to the sensory-motor function of laminae III/IV in Vc.

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