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Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination

Authors
  • Mohon, Abu Naser1, 2
  • Getie, Sisay3
  • Jahan, Nusrat4
  • Alam, Mohammad Shafiul4
  • Pillai, Dylan R.1, 2, 5
  • 1 University of Calgary, Department of Microbiology and Infectious Disease, Cumming School of Medicine, Alberta, T2N 4N1, Canada , Alberta (Canada)
  • 2 University of Calgary, Department of Pathology and Laboratory Medicine, Cumming School of Medicine, 9-3535 Research Road NW, 1 W-416, Calgary, AB, T2L2K8, Canada , Calgary (Canada)
  • 3 University of Gondar, Department of Medical Parasitology, School of Biomedical and Laboratory Science, College of Medicine and Health Sciences, Gondar, Ethiopia , Gondar (Ethiopia)
  • 4 International Center for Diarrheal Disease Research, Bangladesh (icddr,b), Emerging Infections and Parasitology Laboratory, Dhaka, 1212, Bangladesh , Dhaka (Bangladesh)
  • 5 University of Calgary, Department of Medicine, Cumming School of Medicine, Alberta, T2N 4N1, Canada , Alberta (Canada)
Type
Published Article
Journal
Malaria Journal
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Oct 16, 2019
Volume
18
Issue
1
Identifiers
DOI: 10.1186/s12936-019-2979-4
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundMalaria elimination requires diagnostic methods able to detect parasite levels well below what is currently possible with microscopy and rapid diagnostic tests. This is particularly true in surveillance of malaria at the population level that includes so-called “asymptomatic” individuals.MethodsThe development of the first ultrasensitive loop mediated amplification method capable of detecting malaria from both whole blood and dried blood spots (DBS) is described. The 18S rRNA and corresponding genes that remain stable on DBS for up to 5 months are targeted.ResultsIn the case of Plasmodium falciparum, lower limits of detection of 25 parasite/mL and 50–100 parasite/mL from whole blood and DBS were obtained, respectively. A sensitivity of 97.0% (95% CI 82.5–99.8) and specificity of 99.1% (95% CI 97.6–99.7) was obtained for the detection of all species in asymptomatic individuals from Africa and Asia (n = 494).ConclusionThis tool is ideally suited for low middle-income countries where malaria is endemic and ultrasensitive surveillance of malaria is highly desirable for elimination.

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