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Ultrasensitive electrochemiluminescence detection of DNA based on nanoporous gold electrode and [email protected] nanocrystal composites as labels.

Authors
  • Yan, Mei
  • Zhang, Meng
  • Ge, Shenguang
  • Yu, Jinghua
  • Li, Meng
  • Huang, Jiadong
  • Liu, Su
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Jul 21, 2012
Volume
137
Issue
14
Pages
3314–3320
Identifiers
DOI: 10.1039/c2an35267b
PMID: 22662322
Source
Medline
License
Unknown

Abstract

A sensitive electrochemiluminescence (ECL) DNA biosensor based on nanoporous gold (NPG) electrode and [email protected] nanocrystals (CNCs) composites is developed. The CNCs were obtained simply by electrooxidation with abundant carboxyl groups at their surfaces. The NPG can be easily prepared by a selective dissolution of silver from silver-gold alloy in nitric acid, which has free-standing noble metal membranes with controllable three-dimensional (3D) porosity. The PdCu bimetallic nanocomposites with hierarchically hollow structures were fabricated through a simple replacement reaction using dealloyed nanoporous copper (NPC) as both a template and reducing agent. Structure characterization was obtained by means of transmission electron microscope (TEM) and scanning electron microscope (SEM) images. The [email protected] composites exhibit 6 times higher ECL intensity than the pure CNC-labeled reporter DNA. Taking advantage of dual-amplification effects of the developed probe, a limit of detection as low as 18 aM can be achieved and the assay exhibits excellent selectivity for single-mismatched DNA detection even in human serum. The proposed ECL based method should have wide applications in diagnosis of genetic diseases due to its simplicity, low cost, and high sensitivity at extremely low concentrations.

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