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A two-dimensional immunomagnetic nano-net for the efficient isolation of circulating tumor cells in whole blood.

Authors
  • Lai, Chian-Hui1
  • Tsai, Wen-Sy2
  • Yang, Muh-Hwa3
  • Chou, Teh-Ying4
  • Chang, Ying-Chih5
  • 1 Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan. [email protected] and Genomics Research Center, Academia Sinica, Taipei, Taiwan. [email protected] [email protected] and Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan. , (Taiwan)
  • 2 Graduate Institute of Clinical Medical Science, Chang-Gung University, Taoyuan, Taiwan and Division of Colon and Rectal Surgery, Chang-Gung Memorial Hospital, Taoyuan, Taiwan. , (Taiwan)
  • 3 Genomics Research Center, Academia Sinica, Taipei, Taiwan. [email protected] [email protected] and Genome Research Center, National Yang-Ming University, Taipei, Taiwan and Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan and Division of Medical Oncology, Department of Oncology, Taipei Veterans General Hospital, Taipei, Taiwan. , (Taiwan)
  • 4 Division of Thoracic Oncology, Department of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan and Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. , (Taiwan)
  • 5 Genomics Research Center, Academia Sinica, Taipei, Taiwan. [email protected] [email protected] and Department of Chemical Engineering, Stanford University, Stanford, California, USA. , (Taiwan)
Type
Published Article
Journal
Nanoscale
Publisher
The Royal Society of Chemistry
Publication Date
Nov 28, 2019
Volume
11
Issue
44
Pages
21119–21127
Identifiers
DOI: 10.1039/c9nr06256d
PMID: 31538997
Source
Medline
Language
English
License
Unknown

Abstract

An immunomagnetic "nano-net" was designed and synthesized for specifically capturing rare cells of interest from mixtures. The nano-net, [email protected], consists of conjugated antibody molecules on a lipid coated magnetic nanoparticle-graphene oxide sheet complex. The magnetism, chemical composition, and the morphology of the construct and its precursors were characterized by SQUID, FTIR, TGA, DLS and SEM, to confirm the feasibility of the synthetic steps and the resulting properties suitable for solution phase immuno-recognition for cell capture. When applied to capturing circulating tumor cells (CTCs) in oral, colon and lung cancer clinical patients' blood samples, the nano-net construct exhibited far superior ability whereas conventional immunomagnetic beads in some cases were unable to capture any CTCs, even by increasing the bead concentration. Confocal images showed that the nano-net wrapped around the CTCs while the immunomagnetic beads attached them with point contacts. A stable, patch-like multivalent matrix nano-net was demonstrated to tackle the shortcomings of single point contact of immunomagnetic beads to the target cell. This strategy is universal for any cell separation in complex fluids.

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