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Two-temperature formalin fixation preserves activation states efficiently.

Authors
  • Chafin, David
Type
Published Article
Journal
Recent results in cancer research. Fortschritte der Krebsforschung. Progrès dans les recherches sur le cancer
Publication Date
Jan 01, 2015
Volume
199
Pages
107–117
Identifiers
DOI: 10.1007/978-3-319-13957-9_11
PMID: 25636434
Source
Medline
License
Unknown

Abstract

Modern pathology is built around the principle of preserving tissues such that the in vivo molecular status is maintained at levels representative of the disease state. Tissues are immersed in a solution of fixative which slowly inactivates biological activities, thus preserving the sample. Further processing ultimately allows the tissue to be embedded into wax for thin sectioning and staining for interpretation microscopically. Every year, around 7 billion tissue samples are submitted for processing in the United States alone. With this huge workload, histology laboratories are looking for faster methods of performing fixation, which currently require from several hours to days to complete. Ideally, this procedure could be standardized and would be quicker with better preservation over a wide range of biologically relevant molecules.

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