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Two-photon fluorescence bioimaging with an all-semiconductor laser picosecond pulse source.

Authors
  • 1
Type
Published Article
Journal
Optics letters
Publication Date
Volume
32
Issue
18
Pages
2726–2728
Identifiers
PMID: 17873949
Source
Medline

Abstract

We have demonstrated successful two-photon excitation fluorescence bioimaging using a high-power pulsed all-semiconductor laser. Toward this purpose, we developed a pulsed light source consisting of a mode-locked laser diode and a two-stage diode laser amplifier. This pulsed light source provided optical pulses of 5 ps duration and having a maximum peak power of over 100 W at a wavelength of 800 nm and a repetition frequency of 500 MHz.

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