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Two-photon excited hemoglobin fluorescence.

Authors
  • Zheng, Wei
  • Li, Dong
  • Zeng, Yan
  • Luo, Yi
  • Qu, Jianan Y
Type
Published Article
Journal
Biomedical Optics Express
Publisher
The Optical Society
Publication Date
Jan 01, 2010
Volume
2
Issue
1
Pages
71–79
Identifiers
DOI: 10.1364/BOE.2.000071
PMID: 21326637
Source
Medline
Keywords
License
Unknown

Abstract

We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels.

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