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Two-photon excited hemoglobin fluorescence.

Authors
Type
Published Article
Journal
Biomedical Optics Express
2156-7085
Publisher
The Optical Society
Publication Date
Volume
2
Issue
1
Pages
71–79
Identifiers
DOI: 10.1364/BOE.2.000071
PMID: 21326637
Source
Medline
Keywords
  • (170.0170) Medical Optics And Biotechnology
  • (170.2520) Fluorescence Microscopy
  • (180.4315) Nonlinear Microscopy
  • (300.6410) Spectroscopy, Multiphoton
  • (300.6500) Spectroscopy, Time-Resolved

Abstract

We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels.

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