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Two Penicillium camembertii mutants affected in the production of cyclopiazonic acid.

Authors
Type
Published Article
Journal
Applied and Environmental Microbiology
0099-2240
Publisher
American Society for Microbiology
Publication Date
Volume
56
Issue
11
Pages
3587–3590
Identifiers
PMID: 2268164
Source
Medline

Abstract

Penicillium camembertii was mutated and screened for cyclopiazonic acid-negative mutants. With a simple and rapid mini-extraction method for detection of cyclopiazonic acid production, we were able to isolate two strains which were affected in the production of this metabolite. One strain had completely lost the ability to synthesize detectable amounts of this secondary metabolite, whereas the other mutant produced 50 to 100 times less cyclopiazonic acid than the wild type. Also, the former strain had a changed morphology compared with the wild type. This morphological alteration appears to be coupled to the inability to produce cyclopiazonic acid because morphological revertants were able to synthesize cyclopiazonic acid to a level comparable to the wild type. The second mutant accumulated a new metabolite which was detectable by two-dimensional thin-layer chromatography. This new metabolite, however, appears not to be a direct precursor of cyclopiazonic acid.

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