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A two-dimensional protein map of Caenorhabditis elegans.

Authors
  • Schrimpf, S P
  • Langen, H
  • Gomes, A V
  • Wahlestedt, C
Type
Published Article
Journal
Electrophoresis
Publication Date
Apr 01, 2001
Volume
22
Issue
6
Pages
1224–1232
Identifiers
PMID: 11358149
Source
Medline
License
Unknown

Abstract

A protein map of Caenorhabditis elegans was constructed by using two-dimensional gel electrophoresis followed by peptide mass fingerprinting. A whole worm extract of a mixed population was separated on immobilized pH gradient strips 4-7 L, 3-10 NL, 6-11 L and 12% sodium dodecyl sulfate-polyacrylamide gel eletrophoresis (SDS-PAGE) gels. Gels were stained with colloidal Coomassie blue and 286 spots representing 152 proteins were subsequently identified by matrix-assisted laser desorption/ionization-mass spectrometry after in-gel digestion with trypsin. Most of the identified proteins with known cellular function were enzymes related to carbohydrate and lipid metabolism, or structural proteins with subcellular locations in the cytoplasm, mitochondria or cytoskeleton.

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