Affordable Access

deepdyve-link deepdyve-link
Publisher Website

Two dimensional NMR spectroscopic approaches for exploring plant metabolome: A review.

Authors
Type
Published Article
Journal
Journal of advanced research
Publication Date
Volume
6
Issue
1
Pages
3–15
Identifiers
DOI: 10.1016/j.jare.2014.10.003
PMID: 25685540
Source
Medline
Keywords
License
Unknown

Abstract

Today, most investigations of the plant metabolome tend to be based on either nuclear magnetic resonance (NMR) spectroscopy or mass spectrometry (MS), with or without hyphenation with chromatography. Although less sensitive than MS, NMR provides a powerful complementary technique for the identification and quantification of metabolites in plant extracts. NMR spectroscopy, well appreciated by phytochemists as a particularly information-rich method, showed recent paradigm shift for the improving of metabolome(s) structural and functional characterization and for advancing the understanding of many biological processes. Furthermore, two dimensional NMR (2D NMR) experiments and the use of chemometric data analysis of NMR spectra have proven highly effective at identifying novel and known metabolites that correlate with changes in genotype or phenotype. In this review, we provide an overview of the development of NMR in the field of metabolomics with special focus on 2D NMR spectroscopic techniques and their applications in phytomedicines quality control analysis and drug discovery from natural sources, raising more attention at its potential to reduce the gap between the pace of natural products research and modern drug discovery demand.

Statistics

Seen <100 times