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Turn-On Fluorescence Detection of Apoptotic Cells Using a Zinc(II)-Dipicolylamine-Functionalized Poly(diacetylene) Liposome

Authors
  • Cho, YS
  • Kim, KM
  • Lee, D
  • Kim, WJ
  • Ahn, KH
Publication Date
Feb 27, 2013
Source
[email protected]
Keywords
License
Unknown
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Abstract

A liposome-based fluorescence sensing system for apoptotic cells has been developed from stimuli-responsive poly(diacetylene)-liposomes for the first time. The combination of the liposome components, a phosphatidylserine-binding ZnII-dipicolylamine component and an alcohol-terminated component in the ratio of 2:1, has led to an efficient detection system for apoptotic cells, as demonstrated by confocal fluorescence microscopy and FACS analysis. The liposome shows a color change from blue to reddish purple and emits fluorescence in the turn-on mode upon interaction with phosphatidylserine. The present system thus avoids the washing steps required for always-on-type sensing systems. / X / 1 / 26 / 24

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