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A tumor suppressor homolog, AtPTEN1, is essential for pollen development in Arabidopsis.

Authors
  • Gupta, Rajeev
  • Ting, Julie T L
  • Sokolov, Lubomir N
  • Johnson, Sheila A
  • Luan, Sheng
Type
Published Article
Journal
The Plant cell
Publication Date
Oct 01, 2002
Volume
14
Issue
10
Pages
2495–2507
Identifiers
PMID: 12368500
Source
Medline
License
Unknown

Abstract

Although it is well known that Tyr phosphatases play a critical role in signal transduction in animal cells, little is understood of the functional significance of Tyr phosphatases in higher plants. Here, we describe the functional analysis of an Arabidopsis gene (AtPTEN1) that encodes a Tyr phosphatase closely related to PTEN, a tumor suppressor in animals. The recombinant AtPTEN1 protein, like its homologs in animals, is an active phosphatase that dephosphorylates phosphotyrosine and phosphatidylinositol substrates. RNA gel blot analysis and examination of promoter-reporter constructs in transgenic Arabidopsis plants revealed that the AtPTEN1 gene is expressed exclusively in pollen grains during the late stage of development. Suppression of AtPTEN1 gene expression by RNA interference caused pollen cell death after mitosis. We conclude that AtPTEN1 is a pollen-specific phosphatase and is essential for pollen development.

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