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Tumor expression of human chorionic gonadotropin beta mRNA and prognosis of prostate cancer treated by radical prostatectomy.

Authors
  • Lintula, Susanna1
  • Mirtti, Tuomas2, 3
  • Rannikko, Antti4
  • Bützow, Anna2
  • Lempiäinen, Anna1
  • Stenman, Jakob3, 5, 6
  • Stenman, Ulf-Håkan1
  • Hotakainen, Kristina1
  • 1 Department of Clinical Chemistry, University of Helsinki , Helsinki , Finland. , (Finland)
  • 2 Institute for Molecular Medicine Finland (FIMM), University of Helsinki , Helsinki , Finland. , (Finland)
  • 3 Department of Pathology (HUSLAB Laboratory Services), Helsinki University Hospital , Helsinki , Finland. , (Finland)
  • 4 Department of Urology, Helsinki University Hospital , Helsinki , Finland. , (Finland)
  • 5 Department of Pediatric Surgery, Karolinska University Hospital , Stockholm , Sweden. , (Sweden)
  • 6 Department of Women's and Children's Health, Karolinska Institutet , Stockholm , Sweden. , (Sweden)
Type
Published Article
Journal
Scandinavian Journal of Clinical and Laboratory Investigation
Publisher
Informa UK (Taylor & Francis)
Publication Date
Oct 01, 2019
Volume
79
Issue
6
Pages
424–430
Identifiers
DOI: 10.1080/00365513.2019.1639214
PMID: 31294620
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The beta subunit of human chorionic gonadotropin (hCGβ) is encoded by six genes (CGB) classified as type I and type II. CGB mRNA is produced in large amounts by trophoblastic tissues and in small amounts by several cancerous tissues including prostate cancer and by a few benign tissues, including the prostate. Quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was used to study the expression levels of all CGB mRNAs together (total CGB mRNA) and the two types of CGB mRNA separately in non-cancerous (n = 74) and cancerous prostatic tissue obtained by radical prostatectomy (n = 193). RNA was isolated from formalin-fixed paraffin-embedded (FFPE) samples and mRNA levels of CGB were correlated with disease-specific survival. Total CGB mRNA concentrations were significantly lower (p < .0001) in cancerous than non-cancerous prostatic tissue. Separate analysis of type I CGB and type II CGB mRNA showed that both type I CGB (p < .0001) and type II CGB mRNA (p = .007) are lower in cancerous tissue than in non-cancerous tissue. Low type II CGB mRNA level in cancerous tissue was associated with shorter cancer-specific survival (p = .001) of prostate cancer patients treated by radical prostatectomy.

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