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Trypsinized osteoclast-like multinucleated cells formed in rat bone marrow cultures efficiently form resorption lacunae on dentine.

Authors
  • Hata, K1
  • Kukita, T
  • Akamine, A
  • Kukita, A
  • Kurisu, K
  • 1 Department of Periodontics and Endodontics, Faculty of Dentistry, Kyushu University, Fukuoka, Japan. , (Japan)
Type
Published Article
Journal
Bone
Publisher
Elsevier
Publication Date
Jan 01, 1992
Volume
13
Issue
2
Pages
139–146
Identifiers
PMID: 1576009
Source
Medline
License
Unknown

Abstract

Rat bone marrow cultures containing 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3] formed multinucleated cells (MNCs) that had many characteristics of osteoclasts. These MNCs, which have a tartrate-resistant acid phosphatase (TRAP) activity, could be classified into two morphological types: one type had smooth cellular margins (smooth-margined MNCs) and the other type had irregular spike-like margins (stellate MNCs). When bone marrow cells depleted of authentic osteoclasts were seeded and cultured on dentine slices, only low numbers of resorption lacunae could be detected. However, when preformed MNCs were detached by trypsinization and replated on dentine slices, numerous resorption lacunae were observed by scanning electron microscopy on these slices. Formation of lacunae occurred reproducibly during the five to ten days of culture. We also examined the effect of retinoic acid on TRAP-positive MNC formation in this bone marrow culture system. Although RA inhibited total TRAP-positive MNC formation, it increased the ratio of stellate MNCs to smooth-margined MNC, suggesting that RA may have the ability to regulate the formation of active osteoclasts.

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