Triton X-100-treated virus-based ELLA demonstrates discordant antigenic evolution of influenza B virus hemagglutinin and neuraminidase
- Authors
- Type
- Published Article
- Journal
- Journal of Virology
- Publisher
- American Society for Microbiology
- Publication Date
- Oct 03, 2024
- Volume
- 98
- Issue
- 10
- Identifiers
- DOI: 10.1128/jvi.01186-24
- Source
- ASM Journals
- Keywords
- License
- Yellow
Abstract
Influenza B viruses (IBVs) contribute to annual epidemics and may cause severe disease, especially in children. Consequently, several approaches are being explored to improve vaccine efficacy, including the addition of neuraminidase (NA). Antigen selection and assessment of serological responses will require a reliable serological assay to specifically quantify NA inhibition (NI). Although such assays have been assessed for influenza A viruses (IAVs), this has not been done of influenza B viruses. Our study identifies a readily applicable strategy to measure the inhibitory activity of neuraminidase-specific antibodies against influenza B virus without interference from anti-hemagglutinin (anti-HA) antibodies. This will aid broader serological assessment of influenza B virus-specific antibodies and antigenic characterization of the influenza B virus neuraminidase.
