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tris(Hydroxymethyl)aminomethane: An artifact detected as an aminotetrose by gas chromatography/mass spectrometry

Authors
  • Robertson, Nancy P.
  • Cain, George D.
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier BV
Publication Date
Jan 01, 1985
Volume
146
Issue
1
Pages
103–107
Identifiers
DOI: 10.1016/0003-2697(85)90402-6
Source
Elsevier
Keywords
License
Unknown

Abstract

The monosaccharide content of the ethanol-soluble moiety of crude polysaccharide fractions of the tapeworm Hymenolepis diminuta and the blood fluke Schistosoma mansoni was investigated by gas-liquid chromatography of alditol acetate derivatives. A prominent, unusual peak was found, and mass spectrometry suggested identification as an aminotetrose, a fourcarbon aminosugar reported only once in the past 15 years [D. A. Cumming C. G. Hellerquist, and O. Touster (1981), J. Biol. Chem. 256, 7723–7726]. The unknown molecule was found to be tris(hydroxymethyl)aminomethane (Tris), whose acetylated derivative has a mass spectrum identical to that of an aminotetrose. The source of the Tris was traced to buffers used in conjunction with polysaccharide isolation.

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