Affordable Access

deepdyve-link
Publisher Website

Triintsin, a human pathogenic fungus-derived defensin with broad-spectrum antimicrobial activity.

Authors
  • Shen, Bingzheng1
  • Song, Jinchun2
  • Zhao, Yonghui3
  • Zhang, Yaoyun3
  • Liu, Gaomin3
  • Li, Xueke3
  • Guo, Xingchen3
  • Li, Wenxin4
  • Cao, Zhijian5
  • Wu, Yingliang6
  • 1 State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China; Department of pharmacy, Renmin Hospital, Wuhan University, Wuhan 430060, China. , (China)
  • 2 Department of pharmacy, Renmin Hospital, Wuhan University, Wuhan 430060, China. , (China)
  • 3 State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China. , (China)
  • 4 State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China; Bio-drug Research Center, Wuhan University, Wuhan 430072, China. , (China)
  • 5 State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China; Bio-drug Research Center, Wuhan University, Wuhan 430072, China; Hubei Province Engineering and Technology Research, Center for Fluorinated Pharmaceuticals, Wuhan University, Wuhan 430072, China. Electronic address: [email protected] , (China)
  • 6 State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China; Bio-drug Research Center, Wuhan University, Wuhan 430072, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Peptides
Publication Date
Sep 01, 2018
Volume
107
Pages
61–67
Identifiers
DOI: 10.1016/j.peptides.2018.08.003
PMID: 30102941
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Since there is a symbiotic and competitive relationship between microorganisms in the same ecological niche, fungal defensins have been found to be important resources for antimicrobial peptides. Here, a fungal defensin, triintsin, was characterized in a clinical isolate of Trichophyton interdigitale from a patient with onychomycosis. The comparison of its genomic and mRNA sequences showed the gene organization and structure of three coding exons separated by two introns. The precursor peptide of triintsin contained 85 amino acid residues, which were composed of three parts including an N-terminal signal domain of 21 residues, a pro-peptide of 47 residues that ended at lysine-arginine and a mature peptide of 38 residues at the C-terminus. The 3D-structure established by homology modeling revealed that triintsin presented a representative typical cysteine-stabilized α-helical and β-sheet fold. The reductive linear peptide of triintsin was obtained by chemical synthesis. After cyclization to form three pairs of disulfide bonds, the oxidative-type peptide displayed broad-spectrum antimicrobial activity against both gram-positive and gram-negative bacteria but also showed anti-fungal activity. Moreover, triintsin can effectively inhibit the growth of clinical strains. Altogether, the peptide is a human pathogenic fungus-derived defensin with broad-spectrum antimicrobial activity. Copyright © 2018 Elsevier Inc. All rights reserved.

Report this publication

Statistics

Seen <100 times