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Treatment of Cells and Tissues with Chromate Maximizes Mitochondrial 2Fe2S EPR Signals

Authors
  • antholine, william e.
  • vasquez-vivar, jeannette
  • quirk, brendan j.
  • whelan, harry t.
  • kei, pui
  • park, jong-in
  • myers, charles r.
Publication Date
Mar 06, 2019
Source
MDPI
Keywords
Language
English
License
Green
External links

Abstract

however, the EPR signals for N2, the terminal cluster in Complex I, and N4, the cluster preceding the terminal cluster, decrease upon adding chromate. More surprising to us, the EPR signals for N3, the cluster preceding the 2Fe2S cluster in Complex I, also decrease upon adding chromate. Moreover, this method is used to obtain the concentration of the 2Fe2S clusters in white blood cells where the 2Fe2S signal is mostly oxidized before treatment with chromate and becomes reduced and EPR detectable after treatment with chromate. The increase of the g = 1.94 2Fe2S EPR signal upon the addition of chromate can thus be used to obtain the relative steady-state concentration of the 2Fe2S clusters and steady-state concentration of Complex I and/or Complex II in mitochondria.

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