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A transwell assay that excludes exosomes for assessment of tunneling nanotube-mediated intercellular communication.

Authors
  • Thayanithy, Venugopal1, 2
  • O'Hare, Patrick1
  • Wong, Phillip1
  • Zhao, Xianda3
  • Steer, Clifford J4, 5
  • Subramanian, Subbaya3
  • Lou, Emil6, 7
  • 1 Department of Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota, Mayo Mail Code 480, 420 Delaware Street SE, Minneapolis, MN, 55455, USA.
  • 2 Present Address: Molecular Diagnostics Laboratory, University of Minnesota Medical Center, Fairview, 420 Delaware St SE, MMC 198, Minneapolis, MN, 55455, USA.
  • 3 Department of Surgery, University of Minnesota, Minneapolis, MN, 55455, USA.
  • 4 Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Minnesota, Minneapolis, MN, 55455, USA.
  • 5 Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, 55455, USA.
  • 6 Department of Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota, Mayo Mail Code 480, 420 Delaware Street SE, Minneapolis, MN, 55455, USA. [email protected]
  • 7 Graduate Faculty, Department of Integrative Biology and Physiology, University of Minnesota, Minneapolis, MN, 55455, USA. [email protected]
Type
Published Article
Journal
Cell communication and signaling : CCS
Publication Date
Nov 13, 2017
Volume
15
Issue
1
Pages
46–46
Identifiers
DOI: 10.1186/s12964-017-0201-2
PMID: 29132390
Source
Medline
Keywords
License
Unknown

Abstract

This validated assay incorporates several steps that can be taken to quantifiably control for extracellular vesicles in order to perform studies focused on TNT-selective communication.

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