Affordable Access

Access to the full text

Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach

Authors
  • Leichtfried, Thomas1
  • Reisenzein, Helga1
  • Steinkellner, Siegrid2
  • Gottsberger, Richard A.1
  • 1 Austrian Agency for Health and Food Safety, Vienna, 1220, Austria , Vienna (Austria)
  • 2 University of Natural Resources and Life Sciences, Tulln an der Donau, 3430, Austria , Tulln an der Donau (Austria)
Type
Published Article
Journal
Archives of Virology
Publisher
Springer-Verlag
Publication Date
Jul 08, 2020
Volume
165
Issue
11
Pages
2665–2671
Identifiers
DOI: 10.1007/s00705-020-04704-5
Source
Springer Nature
License
Green

Abstract

The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification of the viroid, followed by quantification of relative standard curves by RT-qPCR. Our results indicate that ACFSVd is effectively transmitted by grafting, budding and seeds. No transmission has yet been observed to the viroid-inoculated pome fruit species Pyrus sp. and Cydonia sp. ACFSVd was detected in viruliferous aphids (Myzus persicae, Dysaphis plantaginea) and in codling moths (Cydia pomonella). The viroid was also detected systemically in the infected hemiparasitic plant Viscum album subsp. album (mistletoe).

Report this publication

Statistics

Seen <100 times