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Translation in vitro of hepatitis A virus RNA.

Authors
  • Gauss-Müller, V
  • von der Helm, K
  • Deinhardt, F
Type
Published Article
Journal
Virology
Publication Date
Aug 01, 1984
Volume
137
Issue
1
Pages
182–184
Identifiers
PMID: 6089414
Source
Medline
License
Unknown

Abstract

RNA extracted from highly purified hepatitis A virus (HAV) particles was translated in an RNA-dependent, cell-free reticulocyte lysate system, and the products were analyzed by SDS-polyacrylamide gel electrophoresis and compared with proteins translated from RNA of poliovirus. The efficiency of translation in vitro of HAV RNA was comparable to that of poliovirus RNA. Processing of the putative precursor molecules of HAV proteins, studied in pulse-chase experiments, was impaired when compared with that of poliovirus. By radioimmunoprecipitation it was shown that most proteins produced by translation in vitro were related immunologically to the viral structural proteins of the virion.

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