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Transformation of Kluyveromyces fragilis.

Authors
  • Das, S
  • Kellermann, E
  • Hollenberg, C P
Type
Published Article
Journal
Journal of bacteriology
Publication Date
Jun 01, 1984
Volume
158
Issue
3
Pages
1165–1167
Identifiers
PMID: 6327630
Source
Medline
License
Unknown

Abstract

For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment by alkali cations and with the Kmr gene as the selective marker, a wild-type strain of K. fragilis was transformed to resistance against the antibiotic G418 . In the transformed cell the plasmid replicates autonomously. The same plasmid could also be used to transform S. cerevisiae trp1 mutant to Trp+. Thus, KARS2 of K. lactis enables the vector to replicate in K. fragilis, K. lactis, and S. cerevisiae, whereas ARS1 of S. cerevisiae allows autonomous replication only in S. cerevisiae.

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