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[Transformation of human corneal endothelial cells by micro- injection of oncogenes].

Authors
  • Zavizion, B A
  • Pistsov, M Iu
  • Bergel'son, S M
  • Miroshnichenko, O I
  • Trakht, I N
Type
Published Article
Journal
Biulleten' eksperimental'noĭ biologii i meditsiny
Publication Date
Apr 01, 1990
Volume
109
Issue
4
Pages
395–398
Identifiers
PMID: 2386837
Source
Medline
License
Unknown

Abstract

Human corneal endothelial cells (HCEC) were transfected with some cloned oncogenes. The direct microinjection of either early region (E1) genes of monkey (SA7) and human (Ad5) adenoviruses or Ha-ras oncogen in conjunction with the Ad5 Ela-gene into embryonic HCEC nuclei was shown to result in immortalization of these cells. 3 independent immortalized HCEC lines were established in their growth and morphological properties were studied. These properties were very similar to those of primary HCEC, but unlike primary HCEC the immortalized cells didn't need the endothelial cell growth factor.

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