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Transfer of pyrene-dietherphosphatidylcholine to serum lipoproteins.

Authors
  • Gorges, R
  • Prenner, E
  • Kostner, G M
  • Paltauf, F
  • Hermetter, A
Type
Published Article
Journal
Journal of fluorescence
Publication Date
Dec 01, 1994
Volume
4
Issue
4
Pages
367–368
Identifiers
DOI: 10.1007/BF01881459
PMID: 24233618
Source
Medline
License
Unknown

Abstract

The nonhydrolyzable fluorescent diether analog of phosphatidylcholine, 1-O-hexadecyl-2-0-pyrenedecyl-sn-glycero-3-phosphocholine, has been synthesized as a stable probe for the determination of phospholipid transfer to different lipoprotein classes with potential phospholipase activities. After incubation of total human serum with the new probe at 37°C for 3 hours a characteristic partition equilibrium between the main lipoprotein fractions was observed. The fluorescent lipid was not degraded under these conditions and, therefore, served as a marker for choline glycerophospholipid distribution between and transport to serum lipoproteins.

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