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Transfection of human lymphoblastoid cells with herpes simplex viral DNA.

Authors
  • Miller, G
  • Wertheim, P
  • Wilson, G
  • Robinson, J
  • Geelen, J L
  • van der Noordaa, J
  • van der Eb, A J
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
Feb 01, 1979
Volume
76
Issue
2
Pages
949–953
Identifiers
PMID: 218224
Source
Medline
License
Unknown

Abstract

The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.

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