Tuberculosis is a leading cause of death worldwide and infects thousands of Americans annually. Triclosan (TCS) is used as an oxidative antibacterial in household products such as disinfectants, soaps, detergents, toothpaste. This study presents for the first time, the transcriptomic changes in M. bovis BCG after treatment with 0.1 mM TCS for 10 and 60 min. The results after 10 min exposure to TCS show up-regulations of three groups of functional classes genes in M. bovis BCG. First group are hyc group (hycD, hysP, hysQ, and hysE), which is related to energy production and conversion. Second group is related to oxidation stress of many metabolites, especially, accA2, accD2, fadE12, and fadE13. Third group is associated with ppe family which is distinctive to mycobacteria (ppe3, ppe18, and ppe61). After 60 min exposure the following genes were drastically down-regulated ppe61, hycD hycQ, and bcg0232, which express luxR family two component transcriptional regulators.