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Transcriptome and translatome changes in germinated pollen under heat stress uncover roles of transporter genes involved in pollen tube growth.

Authors
  • Poidevin, Laetitia1
  • Forment, Javier1
  • Unal, Dilek2
  • Ferrando, Alejandro1
  • 1 Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Valencia, Spain. , (Spain)
  • 2 Biotechnology Application and Research Center, and Department of Molecular Biology, Faculty of Science and Letter, Bilecik Seyh Edebali University, Bilecik, Turkey. , (Turkey)
Type
Published Article
Journal
Plant Cell & Environment
Publisher
Wiley (Blackwell Publishing)
Publication Date
Jul 01, 2021
Volume
44
Issue
7
Pages
2167–2184
Identifiers
DOI: 10.1111/pce.13972
PMID: 33289138
Source
Medline
Language
English
License
Unknown

Abstract

Plant reproduction is one key biological process that is very sensitive to heat stress and, as a result, enhanced global warming becomes a serious threat to agriculture. In this work, we have studied the effects of heat on germinated pollen of Arabidopsis thaliana both at the transcriptional and translational level. We have used a high-resolution ribosome profiling technology to provide a comprehensive study of the transcriptome and the translatome of germinated pollen at permissive and restrictive temperatures. We have found significant down-regulation of key membrane transporters required for pollen tube growth by heat, thus uncovering heat-sensitive targets. A subset of the heat-repressed transporters showed coordinated up-regulation with canonical heat-shock genes at permissive conditions. We also found specific regulations at the translational level and we have uncovered the presence of ribosomes on sequences annotated as non-coding. Our results demonstrate that heat impacts mostly on membrane transporters thus explaining the deleterious effects of heat stress on pollen growth. The specific regulations at the translational level and the presence of ribosomes on non-coding RNAs highlights novel regulatory aspects on plant fertilization. © 2020 John Wiley & Sons Ltd.

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