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Transcriptome profiling to identify genes involved in peroxisome assembly and function.

Authors
  • Smith, Jennifer J
  • Marelli, Marcello
  • Christmas, Rowan H
  • Vizeacoumar, Franco J
  • Dilworth, David J
  • Ideker, Trey
  • Galitski, Timothy
  • Dimitrov, Krassen
  • Rachubinski, Richard A
  • Aitchison, John D
Type
Published Article
Journal
The Journal of cell biology
Publication Date
Jul 22, 2002
Volume
158
Issue
2
Pages
259–271
Identifiers
PMID: 12135984
Source
Medline
License
Unknown

Abstract

Yeast cells were induced to proliferate peroxisomes, and microarray transcriptional profiling was used to identify PEX genes encoding peroxins involved in peroxisome assembly and genes involved in peroxisome function. Clustering algorithms identified 224 genes with expression profiles similar to those of genes encoding peroxisomal proteins and genes involved in peroxisome biogenesis. Several previously uncharacterized genes were identified, two of which, YPL112c and YOR084w, encode proteins of the peroxisomal membrane and matrix, respectively. Ypl112p, renamed Pex25p, is a novel peroxin required for the regulation of peroxisome size and maintenance. These studies demonstrate the utility of comparative gene profiling as an alternative to functional assays to identify genes with roles in peroxisome biogenesis.

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