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Transcriptome profiles of the skeletal muscle of mature cows during feed restriction and realimentation

Authors
  • Cunningham-Hollinger, Hannah C.1
  • Kuehn, Larry A.2
  • Cammack, Kristi M.3
  • Hales, Kristin E.2
  • Oliver, William T.2
  • Crouse, Matthew S.2
  • Chen, Celine4
  • Freetly, Harvey C.2
  • Lindholm-Perry, Amanda K.2
  • 1 University of Wyoming,
  • 2 USDA, ARS, U.S. Meat Animal Research Center,
  • 3 South Dakota State University, West River Ag Center,
  • 4 USDA, ARS, Beltsville Human Nutrition Research Center,
Type
Published Article
Journal
BMC Research Notes
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Sep 16, 2021
Volume
14
Identifiers
DOI: 10.1186/s13104-021-05757-8
PMID: 34530907
PMCID: PMC8447676
Source
PubMed Central
Keywords
Disciplines
  • Research Note
License
Unknown

Abstract

Objective Realimentation can compensate for weight loss from poor-quality feedstuffs or drought. Mature cows fluctuate in body weight throughout the year due to nutrient availability. The objective of this study was to determine whether cows that differ in weight gain during realimentation also differ in the abundance of transcripts for enzymes associated with energy utilization in skeletal muscle. Mature cows were subjected to feed restriction followed by ad libitum feed. Skeletal muscle transcriptome expression differences during the two feeding periods were determined from cows with greater (n = 6) and less (n = 6) weight gain during the ad libitum feeding period. Results A total of 567 differentially expressed genes (408 up- and 159 down-regulated) were identified for the comparison of restriction and ad libitum periods (PBonferroni < 0.05). These genes were over-represented in lysosome, aminoacyl-tRNA biosynthesis, and glutathione metabolism pathways. Validation of the expression of five of the genes was performed and four were confirmed. These data suggest that realimentation weight gain for all cows is partially controlled by protein turnover, but oxidative stress and cellular signaling pathways are also involved in the muscle tissue. This dataset provides insight into molecular mechanisms utilized by mature cows during realimentation after a period of low abundance feed. Supplementary Information The online version contains supplementary material available at 10.1186/s13104-021-05757-8.

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