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Transcriptome analysis of putative detoxification genes in the Asian citrus psyllid, Diaphorina citri.

  • Wu, Zhongzhen1
  • Pu, Xinhua1
  • Shu, Benshui1
  • Bin, Shuying1
  • Lin, Jintian1
  • 1 Guangzhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China. , (China)
Published Article
Pest Management Science
Wiley (John Wiley & Sons)
Publication Date
Jun 01, 2020
DOI: 10.1002/ps.5937
PMID: 32483911


The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a notorious pest that transmits the causal agent of huanglongbing (also called citrus greening disease). Resistance to insecticide in this destructive pest poses a serious threat to the citrus industry. To date, no systemic studies on genes coding for detoxification enzymes has been carried out on D. citri. Multiple transcriptomes were generated through deep sequencing of RNA libraries. Candidate genes potentially involved in detoxification including cytochrome P450 monooxygenases (CYPs), glutathione S-transferases (GSTs), and esterases (ESTs) were systematically identified by searching the transcriptomes and a draft genome assembly. A total of 49, 14 and 20 genes were found encoding CYPs, GSTs, and ESTs, respectively, in D. citri. The total numbers of candidate detoxification genes were much smaller than the counterparts reported in other insect species, which may reflect the strict oligophagy of this insect species. Developmental stage- and tissue-specific expression patterns of the identified genes as well as their responses to insecticide treatments identified a small set of genes that could participate in detoxifying plant secondary metabolites and insecticides. Our studies represent the most comprehensive investigation to date on identification, characterization and expression profiling of detoxification genes in D. citri. The information revealed in this study shall be useful in designing strategies to manage this important insect pest. © 2020 Society of Chemical Industry.

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