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Transcriptome analysis of Burkholderia pseudomallei SCV reveals an association with virulence, stress resistance and intracellular persistence.

Authors
  • Al-Maleki, Anis Rageh1
  • Vellasamy, Kumutha Malar2
  • Mariappan, Vanitha2
  • Venkatraman, Gopinath2
  • Tay, Sun Tee2
  • Vadivelu, Jamuna3
  • 1 Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana'a University, Sana'a, Yemen. , (Malaysia)
  • 2 Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. , (Malaysia)
  • 3 Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: [email protected] , (Malaysia)
Type
Published Article
Journal
Genomics
Publisher
Elsevier
Publication Date
Jan 01, 2020
Volume
112
Issue
1
Pages
501–512
Identifiers
DOI: 10.1016/j.ygeno.2019.04.002
PMID: 30980902
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Differences in expression of potential virulence and survival genes were associated with B. pseudomallei colony morphology variants. Microarray was used to investigate B. pseudomallei transcriptome alterations among the wild type and small colony variant (SCV) pre- and post-exposed to A549 cells. SCV pre- and post-exposed have lower metabolic requirements and consume lesser energy than the wild type pre- and post-exposed to A549. However, both the wild type and SCV limit their metabolic activities post- infection of A549 cells and this is indicated by the down-regulation of genes implicated in the metabolism of amino acids, carbohydrate, lipid, and other amino acids. Many well-known virulence and survival factors, including T3SS, fimbriae, capsular polysaccharides and stress response were up-regulated in both the wild type and SCV pre- and post-exposed to A549 cells. Microarray analysis demonstrated essential differences in bacterial response associated with virulence and survival pre- and post-exposed to A549 cells. Copyright © 2019. Published by Elsevier Inc.

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