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The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus

Authors
  • Pelkmans, Jordi F.1
  • Vos, Aurin M.1
  • Scholtmeijer, Karin1, 2
  • Hendrix, Ed2
  • Baars, Johan J. P.2
  • Gehrmann, Thies3
  • Reinders, Marcel J. T.3
  • Lugones, Luis G.1
  • Wösten, Han A. B.1
  • 1 Utrecht University, Microbiology, Department of Biology, Padualaan 8, Utrecht, 3584 CH, The Netherlands , Utrecht (Netherlands)
  • 2 Wageningen University and Research Centre, Plant Breeding, Wageningen, 6700 AJ, The Netherlands , Wageningen (Netherlands)
  • 3 Delft University of Technology, Delft Bioinformatics Lab, Mekelweg 4, Delft, 2628 CD, The Netherlands , Delft (Netherlands)
Type
Published Article
Journal
Applied Microbiology and Biotechnology
Publisher
Springer-Verlag
Publication Date
May 21, 2016
Volume
100
Issue
16
Pages
7151–7159
Identifiers
DOI: 10.1007/s00253-016-7574-9
Source
Springer Nature
Keywords
License
Green

Abstract

The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.

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