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The transcriptional regulation of human arachidonate 12-lipoxygenase gene by NF kappa B/Rel.

Authors
  • Arakawa, T
  • Nakamura, M
  • Yoshimoto, T
  • Yamamoto, S
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Apr 17, 1995
Volume
363
Issue
1-2
Pages
105–110
Identifiers
PMID: 7729529
Source
Medline
License
Unknown

Abstract

As examined by the luciferase assay, a negative regulatory region including the NF kappa B motif was found in the 5'-flanking region of the 12-lipoxygenase gene in human erythroleukemia cells. The negative control was abolished by a site-specific mutation of the NF kappa B motif. Probes including the NF kappa B region gave positive bands upon a gel-shift assay. The bands were super-shifted by antibodies for NF kappa B p50, NF kappa B p65 and c-Rel, and were lost by a NF kappa B competitor DNA. Furthermore, the NF kappa B sequence was protected in DNase I footprinting. Thus, two kinds of heterodimer (p50 and p65; p50 and c-Rel) seemed to control the over-expression of the human 12-lipoxygenase gene.

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