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Transcriptional profiling reveals changes in gene regulation and signaling transduction pathways during temperature stress in wucai ( Brassica campestris L.)

Authors
  • Yuan, Lingyun1, 2, 3
  • Zheng, Yushan1, 2
  • Nie, Libing1, 2
  • Zhang, Liting1, 2
  • Wu, Ying1, 2
  • Zhu, Shidong1, 2, 3
  • Hou, Jinfeng1, 2, 3
  • Shan, Guo Lei1, 2
  • Liu, Tong Kun4
  • Chen, Guohu1, 2
  • Tang, Xiaoyan1, 2
  • Wang, Chenggang1, 2, 3
  • 1 Anhui Agricultural University,
  • 2 Provincial Engineering Laboratory for Horticultural Crop Breeding of Anhui, 130 West of Changjiang Road, Hefei, 230036 Anhui China
  • 3 Wanjiang Vegetable Industrial Technology Institute, Maanshan, 238200 Anhui China
  • 4 Nanjing Agricultural University,
Type
Published Article
Journal
BMC Genomics
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Sep 22, 2021
Volume
22
Identifiers
DOI: 10.1186/s12864-021-07981-9
PMID: 34551703
PMCID: PMC8456696
Source
PubMed Central
Keywords
Disciplines
  • Research
License
Unknown

Abstract

Background Wucai ( Brassica campestris L. ssp. chinensis var. rosularis Tsen) is a cold-tolerant plant that is vulnerable to high temperature. This study explored the response mechanism of wucai to low temperature. In this study, wucai seedlings were treated with different temperatures, including low temperature (LT), high temperature (HT), and a control. Results According to transcriptomics analysis, the number of differentially expressed genes (DEGs) in HT and LT was 10,702 and 7267, respectively, compared with the control. The key genes associated with the physiological response of wucai to the treatments were analyzed. The Kyoto Encyclopedia of Genes and Genomes and Gene Ontology annotations indicated the importance of the photosynthesis and photosynthetic-antenna protein pathways. We found that a high-temperature environment greatly inhibited the expression of important genes in the photosynthetic pathway ( BrLhc superfamily members, PsaD, PsaE, PsaD, PsaD, PsbO, PsbP, PsbQ, PsbR, PsbS, PsbW, PsbY, Psb27, and Psb28 ), whereas low temperature resulted in the expression of certain key genes ( BrLhc superfamily members, Psa F, Psa H, Psb S, Psb H, Psb 28 ). In addition, the wucai seedlings exhibited better photosynthetic performance under low-temperature conditions than high-temperature conditions. Conclusions Based on the above results, we speculate that upon exposure to low temperature, the plants developed higher cold tolerance by upregulating the expression of genes related to photosynthesis. Conversely, high-temperature stress inhibited the expression of pivotal genes and weakened the self-regulating ability of the plants. Supplementary Information The online version contains supplementary material available at 10.1186/s12864-021-07981-9.

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