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Transcriptional patterns of Coffea arabica L. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust.

Authors
  • Baba, Viviane Yumi1, 2
  • Braghini, Masako Toma3
  • Dos Santos, Tiago Benedito2, 4
  • de Carvalho, Kenia2, 5
  • Soares, João Danillo Moura2
  • Ivamoto-Suzuki, Suzana Tiemi2, 6
  • Maluf, Mirian P3, 7
  • Padilha, Lilian3, 7
  • Paccola-Meirelles, Luzia D1, 8
  • Pereira, Luiz Filipe2, 7
  • Domingues, Douglas S2, 6
  • 1 Department of Agronomy, Universidade Estadual de Londrina, Londrina, Paraná, Brazil. , (Brazil)
  • 2 Plant Biotechnology Laboratory, Instituto Agronômico do Paraná, Londrina, Paraná, Brazil. , (Brazil)
  • 3 Centro de Análise e Pesquisa Tecnológica do Agronegócio do Café "Alcides Carvalho," Instituto Agronômico de Campinas, Campinas, São Paulo, Brazil. , (Brazil)
  • 4 Programa de Pós-Graduação em Agronomia, Universidade do Oeste Paulista, Presidente Prudente, São Paulo, Brazil. , (Brazil)
  • 5 Plant Biotechnology Laboratory, Embrapa Soja, Londrina, Paraná, Brazil. , (Brazil)
  • 6 Department of Botany, Instituto de Biociências, São Paulo State University, UNESP, Rio Claro, São Paulo, Brazil. , (Brazil)
  • 7 Plant Breeding, Embrapa Café, Brasília-DF, Brazil. , (Brazil)
  • 8 Department of Agronomy, Universidade Paranaense, Umuarama, Paraná, Brazil. , (Brazil)
Type
Published Article
Journal
PeerJ
Publisher
PeerJ
Publication Date
Jan 01, 2020
Volume
8
Identifiers
DOI: 10.7717/peerj.8320
PMID: 31915587
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study evaluated the transcriptional profile of genes related to nitrogen (N) assimilation in coffee plants susceptible and resistant to rust fungi under N sufficiency and N suppression. For this purpose, we inoculated young coffee leaves with Hemileia vastatrix uredospores and collected them at 0, 12, 24 and 48 hours post-inoculation (HPI) to evaluate the relative expressions of genes encoding cytosolic glutamine synthetase (CaGS1 ), plastid glutamine synthetase (CaGS2 ), nitrate reductase (CaNR), and asparagine synthetase (CaAS). The genes exhibited distinct patterns of transcriptional modulation for the different genotypes and N nutritional regimes. The resistant genotype (I59) presented high levels of transcription in response to pathogen inoculation for CaNR and CaGS1 genes, evaluated under N sufficiency in the initial moments of infection (12 HPI). The gene CaGS1 also showed a peak at 48 HPI. The susceptible genotype (CV99) showed increased transcript rates of CaNR at 12 and 24 HPI in response to rust inoculation. The transcriptional patterns observed for CV99, under N suppression, were high levels for CaAS and CaGS2 at all post-inoculation times in response to coffee leaf rust disease. In addition, CaGS1 was up-regulated at 48 HPI for CV99. Cultivar I59 showed high transcript levels at 12 HPI for CaAS and peaks at 24 and 48 HPI for CaGS2 in inoculated samples. Consequently, total chlorophyl concentration was influenced by N suppression and by rust infection. Regarding enzyme activities in vitro for glutamine synthetase and CaNR, there was an increase in infected coffee leaves (I59) and under N sufficiency. Moreover, CV99 was modulated in both N nutritional regimes for GS activity in response to rust. Our results indicate that N transport genes trigger a differential modulation between genotypes through the action of rust disease. © 2020 Baba et al.

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