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Transcription factors DNA-binding activity in rat thymocytes undergoing apoptosis after heat-shock or dexamethasone treatment.

Authors
  • Sikora, E
  • Grassilli, E
  • Radziszewska, E
  • Bellesia, E
  • Barbieri, D
  • Franceschi, C
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Dec 15, 1993
Volume
197
Issue
2
Pages
709–715
Identifiers
PMID: 8267607
Source
Medline
License
Unknown

Abstract

The onset of apoptosis is generally thought to require the induction of a novel genetic program. Accordingly, we studied the kinetic of DNA-binding activity of several transcription factors in rat thymocytes undergoing apoptosis induced by dexamethasone (DEX) or heat shock (HS) treatment. Here we report that: 1) early activation of AP-1 occurred in both models of apoptosis, even if the intensity of activation and AP-1 complex composition were different and much less evident in HS-treated thymocytes; 2) early NFkB DNA-binding activity was also observed in both types of apoptosis; 3) downregulation of other transcription factors, such as OCT-1 and CREB, with high constitutive activity, was recorded in both models of apoptosis. The fact that some TFs are up-regulated while others are down-regulated suggests that apoptosis is the result of a complex combination of positive and negative signals regulating gene expression.

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