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Transcription factor NF-Y inhibits cell growth and decreases SOX2 expression in human embryonal carcinoma cell line NT2/D1

Authors
  • Mojsin, M.1
  • Topalovic, V.1
  • Marjanovic Vicentic, J.1
  • Stevanovic, M.1
  • 1 University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, Belgrade, 11010, Serbia , Belgrade (Serbia)
Type
Published Article
Journal
Biochemistry (Moscow)
Publisher
Pleiades Publishing
Publication Date
Feb 18, 2015
Volume
80
Issue
2
Pages
202–207
Identifiers
DOI: 10.1134/S0006297915020066
Source
Springer Nature
Keywords
License
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Abstract

Transcription factor NF-Y belongs to the embryonic stem cell transcription factor circuitry due to its role in the regulation of cell proliferation. We investigated the role of NF-Y in pluripotency maintenance using NT2/D1 cells as one of the best-characterized human embryonal carcinoma cell line. We investigated the efficiency of protein transduction and analyzed the effects of forced expression of short isoform of NF-Y A-subunit (NF-YAs) on NT2/D1 cell growth and expression of SOX2. We found that protein transduction is an efficient method for NF-Y overexpression in NT2/D1 cells. Next, we analyzed the effect of NF-YAs overexpression on NT2/D1 cell viability and detected significant reduction in cell growth. The negative effect of NF-YAs overexpression on NT2/D1 cell pluripotency maintenance was confirmed by the decrease in the level of the pluripotency marker SOX2. Finally, we checked the p53 status and determined that the NF-Y-induced inhibition of NT2/D1 cell growth is p53-independent.

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