Affordable Access

deepdyve-link
Publisher Website

Transbilayer Movement of Sphingomyelin Precedes Catastrophic Breakage of Enterobacteria-Containing Vacuoles

Authors
  • Ellison, Cara J.1
  • Kukulski, Wanda2
  • Boyle, Keith B.1
  • Munro, Sean2
  • Randow, Felix1, 3
  • 1 MRC Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Francis Crick Avenue, Cambridge CB2 0QH, UK
  • 2 MRC Laboratory of Molecular Biology, Cell Biology Division, Francis Crick Avenue, Cambridge CB2 0QH, UK
  • 3 University of Cambridge, Department of Medicine, Addenbrooke’s Hospital, Cambridge CB2 0QQ, UK
Type
Published Article
Journal
Current Biology
Publisher
Cell Press
Publication Date
Aug 03, 2020
Volume
30
Issue
15
Pages
2974–2983
Identifiers
DOI: 10.1016/j.cub.2020.05.083
PMID: 32649908
PMCID: PMC7416114
Source
PubMed Central
Keywords
License
Unknown

Abstract

Ellison et al. show that the pore-forming toxin Lysenin can be used as a reporter for detecting sphingomyelin in the cytosolic leaflet of cellular membranes. The breakout of Gram-negative bacteria from vacuoles into the host cytosol follows a precisely choreographed process in which sphingomyelin exposure precedes catastrophic membrane damage.

Report this publication

Statistics

Seen <100 times