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A trans -complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence

Authors
  • Zhang, Xianwen1
  • Liu, Yang1
  • Liu, Jianying2, 3
  • Bailey, Adam L.4
  • Plante, Kenneth S.2, 3, 5
  • Plante, Jessica A.2, 3, 5
  • Zou, Jing1
  • Xia, Hongjie1
  • Bopp, Nathen E.6
  • Aguilar, Patricia V.6
  • Ren, Ping3, 6
  • Menachery, Vineet D.2, 3
  • Diamond, Michael S.4, 7, 8
  • Weaver, Scott C.2, 3, 5, 9
  • Xie, Xuping1, 3
  • Shi, Pei-Yong1, 3, 9, 10
  • 1 Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA
  • 2 Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA
  • 3 Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA
  • 4 Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA
  • 5 World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, TX, USA
  • 6 Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA
  • 7 Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA
  • 8 Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA
  • 9 Sealy Institute for Vaccine Sciences, University of Texas Medical Branch, Galveston, TX, USA
  • 10 Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, USA
Type
Published Article
Journal
Cell
Publisher
Elsevier Inc.
Publication Date
Feb 23, 2021
Identifiers
DOI: 10.1016/j.cell.2021.02.044
PMID: 33691138
PMCID: PMC7901297
Source
PubMed Central
Keywords
License
Unknown

Abstract

Zhang et al. develop a trans- complementation system to produce single-round infectious SARS-CoV-2 that recapitulates authentic viral infection and replication. They then show the system can be safely used at biosafety level 2 for high-throughput antiviral testing.

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