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trans-2-phenylcyclopropylamine is a substrate for and inactivator of horseradish peroxidase.

Authors
  • Sayre, L M
  • Naismith, R T 2nd
  • Bada, M A
  • Li, W S
  • Klein, M E
  • Tennant, M D
Type
Published Article
Journal
Biochimica et Biophysica Acta
Publisher
Elsevier
Publication Date
Sep 05, 1996
Volume
1296
Issue
2
Pages
250–256
Identifiers
PMID: 8814233
Source
Medline
License
Unknown

Abstract

Horseradish peroxidase (HRP) is well known for mediating the electron-transfer oxidation of electron-rich aromatic 'donors' such as phenols and anilines, but has not been described to oxidize aliphatic amines. We here confirm the inability of HRP to oxidize typical aliphatic amines, even those which would exist significantly as free bases at the operative pH. In contrast, trans-2-phenylcyclopropylamine (2-PCPA) is both a substrate (turnover product is cinnamaldehyde) and a time-dependent inactivator of HRP. These activities of 2-PCPA are consistent with either a concerted or rapid sequential one-electron-oxidation/ring-opening to give an intermediate capable of covalent binding to the enzyme. 2-PCPA is the first known example of a simple aliphatic amine which serves as a substrate for HRP under turnover conditions.

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