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Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections.

Authors
  • Küppers, R
  • Zhao, M
  • Hansmann, M L
  • Rajewsky, K
Type
Published Article
Journal
The EMBO journal
Publication Date
Dec 15, 1993
Volume
12
Issue
13
Pages
4955–4967
Identifiers
PMID: 8262038
Source
Medline
License
Unknown

Abstract

Germinal centres are areas of intense B lymphocyte proliferation inside primary B cell follicles in spleen and lymph nodes. Rearranged V genes from single human B cells, isolated from histological sections of two such structures by micromanipulation, were amplified and sequenced. Cells from the follicular mantle were clonally diverse and largely expressed germline V genes. Germinal centres were dominated by a few large B cell clones dispersed throughout these structures and exhibiting intraclonal diversity by ongoing somatic hypermutation. Pronounced counterselection of replacement mutations seen in one of the germinal centres may indicate a late phase of the germinal centre reaction. A polyclonal population of activated B cells expressing unmutated antibodies in the dark zone of the other germinal centre may represent the initial founder cells.

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