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Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections.

Authors
Type
Published Article
Journal
The EMBO journal
Publication Date
Volume
12
Issue
13
Pages
4955–4967
Identifiers
PMID: 8262038
Source
Medline
License
Unknown

Abstract

Germinal centres are areas of intense B lymphocyte proliferation inside primary B cell follicles in spleen and lymph nodes. Rearranged V genes from single human B cells, isolated from histological sections of two such structures by micromanipulation, were amplified and sequenced. Cells from the follicular mantle were clonally diverse and largely expressed germline V genes. Germinal centres were dominated by a few large B cell clones dispersed throughout these structures and exhibiting intraclonal diversity by ongoing somatic hypermutation. Pronounced counterselection of replacement mutations seen in one of the germinal centres may indicate a late phase of the germinal centre reaction. A polyclonal population of activated B cells expressing unmutated antibodies in the dark zone of the other germinal centre may represent the initial founder cells.

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