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Toxigenic Vibrio cholerae O1 in vegetables and fish raised in wastewater irrigated fields and stabilization ponds during a non-cholera outbreak period in Morogoro, Tanzania: an environmental health study

Authors
  • Hounmanou, Yaovi M. G.1, 2
  • Mdegela, Robinson H.1
  • Dougnon, Tamègnon V.2
  • Mhongole, Ofred J.1
  • Mayila, Edward S.3
  • Malakalinga, Joseph1
  • Makingi, George1
  • Dalsgaard, Anders4
  • 1 Sokoine University of Agriculture, Department of Veterinary Medicine and Public Health, Morogoro, Tanzania , Morogoro (Tanzania)
  • 2 University of Abomey-Calavi, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, Abomey-Calavi, Benin , Abomey-Calavi (Benin)
  • 3 National Health Laboratory Quality Assurance and Training Centre (NHLQATC), Dar Es Salaam, Tanzania , Dar Es Salaam (Tanzania)
  • 4 University of Copenhagen, Faculty of Health and Medical Sciences (UC-HEALTH), Copenhagen, Denmark , Copenhagen (Denmark)
Type
Published Article
Journal
BMC Research Notes
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Oct 18, 2016
Volume
9
Issue
1
Identifiers
DOI: 10.1186/s13104-016-2283-0
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundCholera, one of the world’s deadliest infectious diseases, remains rampant and frequent in Tanzania and thus hinders existing control measures. The present study was undertaken to evaluate the occurrence of toxigenic Vibrio cholerae O1 in wastewater, fish and vegetables during a non-outbreak period in Morogoro, Tanzania.MethodsFrom October 2014 to February 2015, 60 wastewater samples, 60 fish samples from sewage stabilization ponds and 60 wastewater irrigated vegetable samples were collected. Samples were cultured for identification of V. cholerae using conventional bacteriological methods. Isolates were confirmed as V. cholerae by detection of the outer membrane protein gene (ompW) using polymerase chain reaction (PCR). Isolates were further tested for antibiotic susceptibility and presence of virulence genes including, cholera enterotoxin gene (ctx), the toxin co-regulated pilus gene (tcpA) and the haemolysin gene (hlyA).ResultsThe prevalence of V. cholerae in wastewater, vegetables and fish was 36.7, 21.7 and 23.3 %, respectively. Two isolates from fish gills were V. cholerae O1 and tested positive for ctx and tcpA. One of these contained in addition the hlyA gene while five isolates from fish intestines tested positive for tcpA. All V. cholerae isolates were resistant to ampicillin, amoxicillin and some to tetracycline, but sensitive to gentamicin, chloramphenicol, and ciprofloxacin.ConclusionsOur results show that toxigenic and drug-resistant V. cholerae O1 species are present and persist in aquatic environments during a non-cholera outbreak period. This is of public health importance and shows that such environments may be important as reservoirs and in the transmission of V. cholerae O1.

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