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Toxicity of Cry1-Class, Cry2Aa, and Vip3Aa19 Bt proteins and their interactions against yellow peach Moth, Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae).

Authors
  • Shwe, Su Mon1
  • Wang, Yueqin1
  • Gao, Zupeng2
  • Li, Xue3
  • Liu, Shen1
  • Bai, Shuxiong1
  • Zhang, Tiantao1
  • He, Kanglai1
  • Wang, Zhenying4
  • 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2, West Yuanmingyuan Road, Beijing 100193, China. , (China)
  • 2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2, West Yuanmingyuan Road, Beijing 100193, China; Engineering Research Center of Natural Enemy Insects/Institute of Biological Control, Jilin Agricultural University, Changchun 130118, China. , (China)
  • 3 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2, West Yuanmingyuan Road, Beijing 100193, China; Plant Protection College, Hebei Agricultural University, Baoding 071000, China. , (China)
  • 4 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2, West Yuanmingyuan Road, Beijing 100193, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Journal of Invertebrate Pathology
Publisher
Elsevier
Publication Date
Nov 26, 2020
Volume
178
Pages
107507–107507
Identifiers
DOI: 10.1016/j.jip.2020.107507
PMID: 33249063
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Transgenic plants expressing insecticidal proteins from the Bacillus thuringiensis (Bt) have provided an effective way to control target pests. However, the toxicity of Bt proteins against yellow peach moth (YPM), Conogethes punctiferalis (Guenée), one of the most serious maize pests in China, has not received much study. Therefore, we performed diet-overlay bioassays to evaluate the insecticidal activities of Cry1Ab, Cry1Ac, Cry1Fa, Cry1Ah, Cry1Ie, Cry2Aa, and Vip3Aa19, as well as the interaction between Cry1-Class, Cry2Aa, and Vip3Aa19 against YPM. Results showed that the LC50 values ranged from 1.08 to 178.12 ng/cm2 (protein/diet). Among these proteins, Cry1Ab and Cry1Ac had lower LC50 values and LC90 values. In YPM bioassays, the combinations of Cry2Aa with Cry1Ac, Cry1Ie, and Cry1Ab showed antagonism while a mixture of Cry2Aa with Cry1Fa and Cry1Ah exhibited synergism. When Vip3Aa19 was combined with Cry proteins, all combinations interacted positively, with variation in synergistic factors (SF). Three ratios 1:1, 1:2, and 2:1 of Cry1Ah and Vip3Aa19 protein combination showed SF values of 5.20, 5.63, and 8.98, respectively. These findings can be applied in the establishment of new pyramided transgenic crops with suitable candidates as well as in resistance management strategies. Copyright © 2020 Elsevier Inc. All rights reserved.

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